Abstract
Detection of Chronic bee paralysis virus (CBPV) is reported for the first time in two species of ants (Camponotus vagus and Formica rufa) and in Varroa destructor. A quantitative real-time PCR (qPCR) method was used to detect and quantify CBPV in infected bees, ants and mites. A minus-strand-specific RT-PCR was used to assess viral replication. These results suggest a new way by which the infection may be spread and other sites of viral persistence in the close apiary environment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Ants / virology*
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Bees / virology*
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Chronic Disease
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Genome, Viral
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Insect Viruses / genetics
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Insect Viruses / isolation & purification*
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Insect Viruses / physiology
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Mites / virology*
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Paralysis / virology
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Polymerase Chain Reaction / methods
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RNA Viruses / genetics
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RNA Viruses / isolation & purification*
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RNA Viruses / physiology
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RNA, Viral / biosynthesis*
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RNA, Viral / genetics
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RNA, Viral / isolation & purification
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, DNA
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Species Specificity
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Virus Replication