Detection of Chronic bee paralysis virus (CBPV) genome and its replicative RNA form in various hosts and possible ways of spread

Olivier Celle; Philippe Blanchard; Violaine Olivier; Frank Schurr; Nicolas Cougoule; Jean-Paul Faucon; Magali Ribière

doi:10.1016/j.virusres.2007.12.011

Detection of Chronic bee paralysis virus (CBPV) genome and its replicative RNA form in various hosts and possible ways of spread

Virus Res. 2008 May;133(2):280-4. doi: 10.1016/j.virusres.2007.12.011. Epub 2008 Feb 19.

Authors

Olivier Celle¹, Philippe Blanchard, Violaine Olivier, Frank Schurr, Nicolas Cougoule, Jean-Paul Faucon, Magali Ribière

Affiliation

¹ Agence Française de Sécurité Sanitaire des Aliments, Les Templiers, Route des Chappes, BP 111, Sophia Antipolis 06902, France.

PMID: 18243390
DOI: 10.1016/j.virusres.2007.12.011

Abstract

Detection of Chronic bee paralysis virus (CBPV) is reported for the first time in two species of ants (Camponotus vagus and Formica rufa) and in Varroa destructor. A quantitative real-time PCR (qPCR) method was used to detect and quantify CBPV in infected bees, ants and mites. A minus-strand-specific RT-PCR was used to assess viral replication. These results suggest a new way by which the infection may be spread and other sites of viral persistence in the close apiary environment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Ants / virology*
Bees / virology*
Chronic Disease
Genome, Viral
Insect Viruses / genetics
Insect Viruses / isolation & purification*
Insect Viruses / physiology
Mites / virology*
Paralysis / virology
Polymerase Chain Reaction / methods
RNA Viruses / genetics
RNA Viruses / isolation & purification*
RNA Viruses / physiology
RNA, Viral / biosynthesis*
RNA, Viral / genetics
RNA, Viral / isolation & purification
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Species Specificity
Virus Replication

Substances

RNA, Viral