Abstract
A tributyltin (TBT) luxAB transcriptional fusion in Escherichia coli revealed that a TBT-activated promoter is located upstream of two cotranscribed orphan genes, ygaV and ygaP. We demonstrate that transcription from the promoter upstream of ygaVP is constitutive in a ygaVP mutant, suggesting that YgaV is an autoregulated, TBT-inducible repressor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acid Anhydride Hydrolases / genetics
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Amino Acid Sequence
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Escherichia coli / genetics*
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Escherichia coli Proteins / genetics*
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Gene Expression Regulation, Bacterial / drug effects*
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Molecular Sequence Data
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Mutation
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Operon*
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Polymerase Chain Reaction
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Promoter Regions, Genetic / genetics
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Repressor Proteins / genetics*
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Sequence Homology, Amino Acid
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Transcription, Genetic / drug effects
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Trialkyltin Compounds / pharmacology*
Substances
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Escherichia coli Proteins
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Repressor Proteins
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Trialkyltin Compounds
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YgaP protein, E coli
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YgaV protein, E coli
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tributyltin
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Acid Anhydride Hydrolases
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RppH protein, E coli