Simultaneous analysis of five antidepressant drugs using direct injection of biofluids in a capillary restricted-access media-liquid chromatography-tandem mass spectrometry system

J Chromatogr A. 2008 May 2;1189(1-2):514-22. doi: 10.1016/j.chroma.2008.02.107. Epub 2008 Mar 7.

Abstract

Direct analysis, with minimal sample pretreatment, of antidepressant drugs, fluoxetine, imipramine, desipramine, amitriptyline, and nortriptyline in biofluids was developed with a total run time of 8 min. The setup consists of two HPLC pumps, injection valve, capillary RAM-ADS-C18 pre-column and a capillary analytical C18 column connected by means of a six-port valve in backflush mode. Detection was performed with ESI-MS/MS and only 1 microm of sample was injected. Validation was adequately carried out using FLU-d(5) as internal standard. Calibration curves were constructed under a linear range of 1-250 ng mL(-1) in plasma, being the limit of quantification (LOQ), determined as 1 ng mL(-1), for all the analytes. With the described approach it was possible to reach a quantified mass sensitivity of 0.3 pg for each analyte (equivalent to 1.1-1.3 fmol), translating to a lower sample consumption (in the order of 10(3) less sample than using conventional methods).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antidepressive Agents / analysis*
  • Antidepressive Agents / chemistry
  • Chromatography, High Pressure Liquid / methods*
  • Isonipecotic Acids / analysis
  • Isonipecotic Acids / chemistry
  • Meperidine / analogs & derivatives
  • Meperidine / analysis
  • Meperidine / chemistry
  • Phenols / analysis
  • Phenols / chemistry
  • Reproducibility of Results
  • Tandem Mass Spectrometry / methods*

Substances

  • Antidepressive Agents
  • Isonipecotic Acids
  • Phenols
  • norketobemidone
  • Meperidine
  • ketobemidone