Objective: To report a case of a successful pregnancy after trophectoderm biopsy and three-probe fluorescent in situ hybridization of a frozen blastocyst.
Design: Techniques and instrumentation.
Setting: A University Medical Center.
Patient(s): Infertility patient desiring trophectoderm biopsy on frozen blastocyst for preimplantation testing, from an IVF cycle at a referring IVF program.
Intervention(s): Frozen blastocysts were thawed the evening before the planned transfer. Trophectoderm biopsy was performed in the morning. The fluorescent in situ hybridization results were obtained the same day; embryo transfer was performed under ultrasound guidance.
Main outcome measurement(s): Serum betahCG and transvaginal ultrasound.
Result(s): Positive betahCG and ongoing pregnancy.
Conclusion(s): Trophectoderm biopsy can be used as a means for testing frozen blastocysts in patients with excess embryos cryopreserved on day 5 or 6 from previously preformed IVF cycles.