Rapid differentiation of methicillin-susceptible Staphylococcus aureus from methicillin-resistant S. aureus and MIC determinations by isothermal microcalorimetry

J Clin Microbiol. 2008 Jun;46(6):2083-7. doi: 10.1128/JCM.00611-08. Epub 2008 Apr 16.

Abstract

In this study, the use of isothermal microcalorimetry (IMC) for differentiation between methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) and MIC determination was evaluated. It was possible to differentiate between MRSA and MSSA within 4 h, whereas the standard method required 24 h. The MICs of cefoxitin were successfully determined for MRSA and MSSA by using IMC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Calorimetry / instrumentation
  • Calorimetry / methods*
  • Cefoxitin / pharmacology
  • Hot Temperature
  • Humans
  • Methicillin / pharmacology*
  • Methicillin Resistance*
  • Microbial Sensitivity Tests / methods
  • Staphylococcal Infections / microbiology
  • Staphylococcus aureus / classification*
  • Staphylococcus aureus / drug effects*
  • Staphylococcus aureus / isolation & purification
  • Time Factors

Substances

  • Anti-Bacterial Agents
  • Cefoxitin
  • Methicillin