Abstract
Klebsiella pneumoniae catabolizes both 4-hydroxyphenylacetic acid and 3-hydroxyphenylacetic acid via meta-cleavage of 3,4-dihydroxyphenylacetic acid, ultimately yielding pyruvate and succinate. The organism can synthesize two hydroxylases catalysing 3,4-dihydroxyphenylacetic acid formation, which differ in substrate specificity, cofactor requirement, kinetics and regulation. Five enzymes sequentially involved in the catabolism of 3,4-dihydroxyphenylacetic acid are encoded on a 7 kbp fragment of the K. pneumoniae chromosome that has been isolated in a recombinant plasmid.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3,4-Dihydroxyphenylacetic Acid / metabolism
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Cloning, Molecular
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Cyclic AMP / metabolism
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Genes, Bacterial
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Klebsiella pneumoniae / enzymology
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Klebsiella pneumoniae / genetics
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Klebsiella pneumoniae / metabolism*
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Mixed Function Oxygenases / genetics
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Mixed Function Oxygenases / metabolism
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Molecular Structure
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Mutation
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Oxidation-Reduction
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Phenylacetates / metabolism*
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Plasmids
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Pyruvates / metabolism
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Pyruvic Acid
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Succinates / metabolism
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Succinic Acid
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Transformation, Bacterial
Substances
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Phenylacetates
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Pyruvates
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Succinates
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3,4-Dihydroxyphenylacetic Acid
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4-hydroxyphenylacetic acid
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Pyruvic Acid
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Succinic Acid
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Cyclic AMP
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Mixed Function Oxygenases
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3-hydroxyphenylacetate 4-monooxygenase
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4-hydroxyphenylacetate 3-monooxygenase
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3-hydroxybenzeneacetic acid