Catabolism of 3- and 4-hydroxyphenylacetic acid by Klebsiella pneumoniae

J Gen Microbiol. 1991 Mar;137(3):621-8. doi: 10.1099/00221287-137-3-621.

Abstract

Klebsiella pneumoniae catabolizes both 4-hydroxyphenylacetic acid and 3-hydroxyphenylacetic acid via meta-cleavage of 3,4-dihydroxyphenylacetic acid, ultimately yielding pyruvate and succinate. The organism can synthesize two hydroxylases catalysing 3,4-dihydroxyphenylacetic acid formation, which differ in substrate specificity, cofactor requirement, kinetics and regulation. Five enzymes sequentially involved in the catabolism of 3,4-dihydroxyphenylacetic acid are encoded on a 7 kbp fragment of the K. pneumoniae chromosome that has been isolated in a recombinant plasmid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3,4-Dihydroxyphenylacetic Acid / metabolism
  • Cloning, Molecular
  • Cyclic AMP / metabolism
  • Genes, Bacterial
  • Klebsiella pneumoniae / enzymology
  • Klebsiella pneumoniae / genetics
  • Klebsiella pneumoniae / metabolism*
  • Mixed Function Oxygenases / genetics
  • Mixed Function Oxygenases / metabolism
  • Molecular Structure
  • Mutation
  • Oxidation-Reduction
  • Phenylacetates / metabolism*
  • Plasmids
  • Pyruvates / metabolism
  • Pyruvic Acid
  • Succinates / metabolism
  • Succinic Acid
  • Transformation, Bacterial

Substances

  • Phenylacetates
  • Pyruvates
  • Succinates
  • 3,4-Dihydroxyphenylacetic Acid
  • 4-hydroxyphenylacetic acid
  • Pyruvic Acid
  • Succinic Acid
  • Cyclic AMP
  • Mixed Function Oxygenases
  • 3-hydroxyphenylacetate 4-monooxygenase
  • 4-hydroxyphenylacetate 3-monooxygenase
  • 3-hydroxybenzeneacetic acid