Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvant

Vaccine. 2008 Oct 16;26(44):5662-7. doi: 10.1016/j.vaccine.2008.07.027. Epub 2008 Sep 21.

Abstract

Mucosal vaccine formulations based on purified recombinant C280 gamma-Intimin and EspB (Escherichia coli secreted protein B) from enterohaemorragic E. coli co-administered with a pegylated derivative of the TLR2/6 agonist MALP-2 (macrophage-activating lipopeptide) as adjuvant were evaluated in BALB/c mice. After intranasal vaccination, strong humoral and cellular immune responses were observed against C280 gamma-Intimin and EspB. Sera of immunized mice inhibit bacterial haemolytic activity in vitro. Antigen-specific T-cell proliferation, IL-4, IL-2 and IFN-gamma producing cells, and secretory IgA were mostly detected in animals receiving MALP-2 as adjuvant. These results suggest that C280 gamma-Intimin and EspB are good candidate antigens to be incorporated into mucosal vaccines against this important pathogen.

MeSH terms

  • Adhesins, Bacterial / immunology*
  • Adjuvants, Immunologic*
  • Administration, Intranasal
  • Animals
  • Antibodies, Bacterial / blood
  • Bacterial Outer Membrane Proteins / immunology*
  • Enterohemorrhagic Escherichia coli / immunology*
  • Escherichia coli Infections / immunology
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / prevention & control
  • Escherichia coli Proteins / immunology*
  • Escherichia coli Vaccines / administration & dosage*
  • Escherichia coli Vaccines / immunology
  • Female
  • Lipopeptides / immunology*
  • Lymphocyte Activation / immunology
  • Mice
  • Mice, Inbred BALB C
  • T-Lymphocytes / immunology
  • Toll-Like Receptor 2 / agonists
  • Toll-Like Receptor 6 / agonists
  • Vaccination

Substances

  • Adhesins, Bacterial
  • Adjuvants, Immunologic
  • Antibodies, Bacterial
  • Bacterial Outer Membrane Proteins
  • EaeB protein, E coli
  • Escherichia coli Proteins
  • Escherichia coli Vaccines
  • Lipopeptides
  • Tlr2 protein, mouse
  • Tlr6 protein, mouse
  • Toll-Like Receptor 2
  • Toll-Like Receptor 6
  • eaeA protein, E coli
  • macrophage stimulatory lipopeptide 2