Abstract
In this report we summarize our experiences with the reaction products of N-hydroxysuccinimide (NHS) esters, which are widely used for chemical cross-linking of lysine residues in proteins. We describe the products, which should be scrutinized during data analysis using customized software when NHS esters are employed for chemical cross-linking. Reaction products of NHS esters were observed not only with lysines, but also with serines, tyrosines, and threonines. This report is intended to be a practical guide for those working in the field of chemical cross-linking and mass spectrometry.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amino Acids / chemistry*
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Cross-Linking Reagents / chemistry*
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Esters / chemistry
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Laminin / chemistry
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Lysine / chemistry
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Molecular Sequence Data
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Peptides / analysis
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Peptides / chemistry*
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Serine / chemistry
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
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Succinimides / chemistry*
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Threonine / chemistry
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Tyrosine / chemistry
Substances
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Amino Acids
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Cross-Linking Reagents
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Esters
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LAMB1 protein, human
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Laminin
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Peptides
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Succinimides
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laminin gamma 1
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Threonine
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Tyrosine
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Serine
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Lysine
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N-hydroxysuccinimide