Expression, purification and characterization of endo-type chitosanase of Aspergillus sp. CJ22-326 from Escherichia coli

Songlin Li; Liang Chen; Chen Wang; Wenshui Xia

doi:10.1016/j.carres.2008.08.032

Expression, purification and characterization of endo-type chitosanase of Aspergillus sp. CJ22-326 from Escherichia coli

Carbohydr Res. 2008 Nov 24;343(17):3001-4. doi: 10.1016/j.carres.2008.08.032. Epub 2008 Sep 9.

Authors

Songlin Li¹, Liang Chen, Chen Wang, Wenshui Xia

Affiliation

¹ State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, 214122 Jiangsu, PR China.

PMID: 18842258
DOI: 10.1016/j.carres.2008.08.032

Abstract

An endo-chitosanase gene was cloned from Aspergillus sp. CJ22-326 and expressed in Escherichia coli. The purified protein showed an endo-chitosanase activity during viscosimetric assay and TLC analysis. The enzyme had higher chitosanolytic activity than previously reported fungal chitosanases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aspergillus / enzymology*
Aspergillus / genetics
Aspergillus fumigatus / enzymology
Aspergillus fumigatus / genetics
Chromatography, Thin Layer
Cloning, Molecular
Escherichia coli / enzymology*
Fungal Proteins / genetics
Fungal Proteins / isolation & purification
Fungal Proteins / metabolism
Glycoside Hydrolases / genetics*
Glycoside Hydrolases / isolation & purification
Glycoside Hydrolases / metabolism
Kinetics
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism
Viscosity

Substances

Fungal Proteins
Recombinant Proteins
Glycoside Hydrolases
chitosanase