Accurate determination of low pK values by (1)H NMR titration

Zoltán Szakács; Gerhard Hägele

doi:10.1016/j.talanta.2003.10.007

Accurate determination of low pK values by (1)H NMR titration

Talanta. 2004 Mar 10;62(4):819-25. doi: 10.1016/j.talanta.2003.10.007.

Authors

Zoltán Szakács¹, Gerhard Hägele

Affiliation

¹ Institut für Anorganische Chemie und Strukturchemie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, D-40225 Düsseldorf, Germany.

PMID: 18969368
DOI: 10.1016/j.talanta.2003.10.007

Abstract

The NMR titration methodology to determine acid dissociation constants in aqueous solutions is extended for pK(a) values between 0 and 2, where potentiometric titrations are no longer applicable. (1)H NMR spectra are acquired for single samples of constant acid concentration (e.g. 0.02M), controlled ionic strength (I=1M with HNO(3)/NaNO(3)) and varying pH. To avoid biased pH readings due to the acid error of the glass electrode, true, concentration-based pH values are deduced by combination of the charge balance equation with information from (1)H NMR chemical shifts of the investigated acid. The method has been tested on histidine (pK(1)=1.83+/-0.02) and yielded the dissociation constant of dichloroacetic acid (pK=1.06+/-0.01) for the first time with good accuracy and precision. Dichloroacetic acid is recommended as an NMR spectroscopical "indicator molecule" for in situ monitoring the pH in strong acidic solutions of other equilibrium systems.