Molecular characterization of the interleukin-8 receptor

Biochem Biophys Res Commun. 1991 Sep 16;179(2):784-9. doi: 10.1016/0006-291x(91)91885-g.

Abstract

Recently a rabbit cDNA (F3R) was characterized as binding and causing calcium mobilization induced by the formyl-methionine-leucine-phenylalanine peptide (fMLP). In the study reported here, cloned DNAs were isolated from rabbit genomic DNA by PCR based on the sequence of F3R. The cloned DNAs have several differences in the DNA sequence compared to the reported F3R sequence that alter the predicted protein sequence. COS-7 cells transfected with these clones in a mammalian expression vector bind human IL-8 with high affinity, but do not bind fMLP. We therefore believe that the cDNAs isolated encode the rabbit IL-8 receptor.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Chemotaxis, Leukocyte / drug effects
  • DNA / analysis*
  • Gene Expression
  • Interleukin-8 / metabolism
  • Molecular Sequence Data
  • N-Formylmethionine Leucyl-Phenylalanine / metabolism
  • Neutrophils / drug effects
  • Polymerase Chain Reaction
  • Rabbits
  • Receptors, Formyl Peptide
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / metabolism
  • Receptors, Interleukin-8A
  • Recombinant Proteins / metabolism
  • Recombinant Proteins / pharmacology
  • Sequence Homology, Nucleic Acid
  • Transfection

Substances

  • Interleukin-8
  • Receptors, Formyl Peptide
  • Receptors, Immunologic
  • Receptors, Interleukin-8A
  • Recombinant Proteins
  • N-Formylmethionine Leucyl-Phenylalanine
  • DNA

Associated data

  • GENBANK/M64052
  • GENBANK/M64053
  • GENBANK/M64054
  • GENBANK/M64055
  • GENBANK/M64056
  • GENBANK/M64057
  • GENBANK/M64058
  • GENBANK/M64059
  • GENBANK/M64060
  • GENBANK/M74240