Biochemical composition of seven small indigenous fresh water fishes namely Magur (Clarias batrachus), Shingi (Heteropneustes fossilis), Koi (Anabas testudineus), Foli (Notopterus notopterus), Royna (Nandas nandas), Taki (Channa punctatus) and Tangra (Mystus vittatus) from the Mouri river Khulna, Bangladesh was studied in order to evaluate their nutritional values. The mean value of protein, fat, moisture and ash content was found as 14.87 +/- 0.63, 7.90 +/- 1.91, 73.49 +/- 0.69 and 3.74 +/- 0.46% in C. batrachus; 17.34 +/- 0.51, 3.45 +/- 0.92, 76.06 +/- 2.24 and 3.15 +/- 0.25% in H. fossilis; 19.63 +/- 0.5, 7.79 +/- 2.73, 69.27 +/- 1.04 and 3.31 +/- 0.83% in A. testudineus; 18.30 +/- 0.79, 4.98 +/- 1.71, 72.68 +/- 1.08 and 5.82 +/- 0.82% in N. notopterus; 16.09 +/- 2.66, 7.34 +/- 0.49, 75.75 +/- 0.78 and 5.19 +/- .029% in N. nandas; 19.13 +/- 2.40, 4.55 +/- 1.18, 70.55 +/- 1.89 and 6.81 +/- 0.94% in C. punctatus and 15.62 +/- 0.32, 7.53 +/- 1.10, 73.99 +/- 3.13 and 6.50 +/- 0.63% in M. vittatus. Considering the result of the present study, it can be concluded that all the species are rich in food value.