Abstract
Using chromatin immunoprecipitation combined with genomic microarrays we have identified targets of No tail (Ntl), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We show that Ntl regulates a downstream network of other transcription factors and identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. We show that the notochord-expressed gene floating head (flh) is a direct transcriptional target of Ntl and that a combination of TBSs in the flh upstream region are required for Ntl-directed expression. Using our genome-scale data we have assembled a preliminary gene regulatory network that begins to describe mesoderm formation and patterning in the early zebrafish embryo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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Body Patterning / genetics
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Cell Lineage
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Conserved Sequence
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Fetal Proteins / genetics
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Fetal Proteins / metabolism*
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Gastrulation / genetics
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Gene Expression Regulation, Developmental
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Gene Regulatory Networks*
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism
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Mesoderm / embryology*
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Molecular Sequence Data
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Muscles / cytology
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Protein Binding
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T-Box Domain Proteins / genetics
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T-Box Domain Proteins / metabolism*
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transcription, Genetic
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Zebrafish / embryology*
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Zebrafish / genetics*
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Zebrafish Proteins / genetics
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Zebrafish Proteins / metabolism
Substances
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Fetal Proteins
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Homeodomain Proteins
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T-Box Domain Proteins
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Transcription Factors
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Zebrafish Proteins
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noto protein, zebrafish
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Brachyury protein