Identification of amino acid substitutions in mutated peptides of nucleoprotein from avian influenza virus

Ning Liu; Kim-Chung Lee; Wenjun Song; Pui Wang; Zongwei Cai; Honglin Chen

doi:10.1016/j.talanta.2009.01.057

Identification of amino acid substitutions in mutated peptides of nucleoprotein from avian influenza virus

Talanta. 2009 Jun 15;78(4-5):1492-6. doi: 10.1016/j.talanta.2009.01.057. Epub 2009 Feb 10.

Authors

Ning Liu¹, Kim-Chung Lee, Wenjun Song, Pui Wang, Zongwei Cai, Honglin Chen

Affiliation

¹ Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, SAR, China.

PMID: 19362222
DOI: 10.1016/j.talanta.2009.01.057

Abstract

Nucleoprotein (NP), the structural component of ribonucleoprotein complex of avian influenza virus, performs multiple essential functions in the regulation of viral RNA synthesis and in the control of nuclear traffic of viral proteins. Mutations have often been found in NP, some of which are relevant to viral survival strategies. In this study, we used nanospray-MS/MS to analyze tryptic digestion of nucleoprotein of avian influenza virus (H5N1) and to identify three mutated peptides. The MS/MS analyses allowed the confident determination of the three mutated amino acid residues F313Y, I194V and V408I/L in the mutated peptides of LLQNSQVYSLIRPNENPAHK, GVGTMVMELVR and ASAGQI/LSVQPTFSVQR, respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Substitution
Animals
Birds
DNA Mutational Analysis
Influenza A Virus, H5N1 Subtype / genetics*
Influenza in Birds / virology*
Mutation, Missense*
Nucleoproteins / genetics*
Peptide Fragments / genetics*
Tandem Mass Spectrometry

Substances

Nucleoproteins
Peptide Fragments