Inhibition of adipocyte differentiation by RORalpha

FEBS Lett. 2009 Jun 18;583(12):2031-6. doi: 10.1016/j.febslet.2009.05.019. Epub 2009 May 18.

Abstract

Here we show that gene expression of the nuclear receptor RORalpha is induced during adipogenesis, with RORalpha4 being the most abundantly expressed isoform in human and murine adipose tissue. Over-expression of RORalpha4 in 3T3-L1 cells impairs adipogenesis as shown by the decreased expression of adipogenic markers and lipid accumulation, accompanied by decreased free fatty acid and glucose uptake. By contrast, mouse embryonic fibroblasts from staggerer mice, which carry a mutation in the RORalpha gene, differentiate more efficiently into mature adipocytes compared to wild-type cells, a phenotype which is reversed by ectopic RORalpha4 restoration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / cytology*
  • Adipocytes / metabolism*
  • Adipogenesis / genetics
  • Adipogenesis / physiology
  • Adult
  • Animals
  • Cell Differentiation / genetics
  • Cell Differentiation / physiology
  • Fatty Acids, Nonesterified / metabolism
  • Gene Expression
  • Glucose / metabolism
  • Humans
  • In Vitro Techniques
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Neurologic Mutants
  • Nuclear Receptor Subfamily 1, Group F, Member 1
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Cytoplasmic and Nuclear / deficiency
  • Receptors, Cytoplasmic and Nuclear / genetics
  • Receptors, Cytoplasmic and Nuclear / metabolism*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Trans-Activators / deficiency
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*

Substances

  • Fatty Acids, Nonesterified
  • Nuclear Receptor Subfamily 1, Group F, Member 1
  • RNA, Messenger
  • RORA protein, human
  • Receptors, Cytoplasmic and Nuclear
  • Recombinant Proteins
  • Trans-Activators
  • Glucose