A depletion strategy for improved detection of human proteins from urine

J Biomol Tech. 2009 Apr;20(2):101-8.

Abstract

With rapidly growing interest in the urine proteome, methods for reducing sample complexity are becoming increasingly important. Depletion strategies for removal of high-abundance proteins from human urine have not been reported. A commercial kit designed for depletion of abundant proteins from plasma was evaluated for removing top proteins from urine of patients with proteinuria. The number of low-abundance proteins identified in urine after depletion increased nearly 2.5-fold.

Keywords: affinity chromatography; protein depletion; proteinuria; proteomics; urine.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Proteins / analysis
  • Blood Proteins / chemistry
  • Blood Proteins / isolation & purification
  • Case-Control Studies
  • Chromatography, Affinity
  • Chromatography, Liquid
  • Electrophoresis, Gel, Two-Dimensional
  • Humans
  • Proteinuria*
  • Proteome / analysis*
  • Proteomics / methods*
  • Reagent Kits, Diagnostic
  • Silver Staining
  • Tandem Mass Spectrometry
  • Ultrafiltration

Substances

  • Blood Proteins
  • Proteome
  • Reagent Kits, Diagnostic