Biotransformation of ginsenoside Rf to Rh1 by recombinant beta-glucosidase

Molecules. 2009 Jun 8;14(6):2043-8. doi: 10.3390/molecules14062043.

Abstract

An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a beta-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (approximately 170 kD) showed the ability to transform ginsenoside Rf into Rh(1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus niger / enzymology
  • Chromatography, High Pressure Liquid
  • Ginsenosides / chemistry
  • Ginsenosides / metabolism*
  • Molecular Structure
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • beta-Glucosidase / genetics
  • beta-Glucosidase / metabolism*

Substances

  • Ginsenosides
  • Recombinant Proteins
  • ginsenoside Rh1
  • beta-Glucosidase
  • ginsenoside Rf