Development and application of quantitative-PCR tools for subgroups of the Roseobacter clade

Appl Environ Microbiol. 2009 Dec;75(23):7542-7. doi: 10.1128/AEM.00814-09. Epub 2009 Oct 2.

Abstract

Specific SYBR green-based quantitative-PCR assays targeting conserved regions in the 16S-23S rRNA internal transcribed spacer regions were developed for five subgroups of the environmentally abundant and biogeochemically active Roseobacter clade of marine bacteria. The assays were applied to field samples demonstrating their utility in investigations of abundant Roseobacter group phylotypes in the environment.

Publication types

  • Evaluation Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacteriological Techniques / methods*
  • Benzothiazoles
  • Biodiversity
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal Spacer / chemistry
  • DNA, Ribosomal Spacer / genetics
  • Diamines
  • Environmental Microbiology
  • Fluorescent Dyes
  • Molecular Sequence Data
  • Organic Chemicals
  • Phylogeny
  • Polymerase Chain Reaction / methods*
  • Quinolines
  • Roseobacter / classification*
  • Roseobacter / genetics*
  • Sequence Analysis, DNA
  • Staining and Labeling / methods

Substances

  • Benzothiazoles
  • DNA, Bacterial
  • DNA, Ribosomal Spacer
  • Diamines
  • Fluorescent Dyes
  • Organic Chemicals
  • Quinolines
  • SYBR Green I

Associated data

  • GENBANK/GQ342302
  • GENBANK/GQ342303
  • GENBANK/GQ342304
  • GENBANK/GQ342305
  • GENBANK/GQ342306
  • GENBANK/GQ342307
  • GENBANK/GQ342308
  • GENBANK/GQ342309
  • GENBANK/GQ342310
  • GENBANK/GQ342311
  • GENBANK/GQ342312
  • GENBANK/GQ342313
  • GENBANK/GQ342314
  • GENBANK/GQ342315
  • GENBANK/GQ342316
  • GENBANK/GQ342317