Determination of catechin diastereomers from the leaves of Byrsonima species using chiral HPLC-PAD-CD

Chirality. 2010 Aug;22(8):726-33. doi: 10.1002/chir.20824.

Abstract

When catechins are found in plant extracts, they are almost always identified as catechin and/or epicatechin probably due to stereoselectivity of the enzymes involved in the biosynthesis of these substances. However, the lack of reports regarding to ent-catechin as well as ent-epicatechin does not necessarily mean that these compounds have not been produced. In fact, most of the previous reports used chromatographic conditions not suitable for such separation. This article describes a simple and reliable analytical HPLC-PAD-CD method for simultaneous determination of catechin diastereomers both in infusions and extracts from the leaves of Byrsonima species. The direct separation of catechin, ent-catechin, epicatechin, and ent-epicatechin was obtained in normal phase by HPLC-PAD-CD using Chiralcel OD-H as chiral stationary phase and n-hexane/ethanol with 0.1% of TFA as mobile phase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catechin / analysis*
  • Catechin / chemistry*
  • Catechin / isolation & purification
  • Chromatography, High Pressure Liquid / methods*
  • Circular Dichroism / methods*
  • Electrodes
  • Linear Models
  • Malpighiaceae / chemistry*
  • Methanol / chemistry
  • Plant Leaves / chemistry*
  • Reproducibility of Results
  • Stereoisomerism

Substances

  • Catechin
  • Methanol