Two-photon single-cell optogenetic control of neuronal activity by sculpted light

Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):11981-6. doi: 10.1073/pnas.1006620107. Epub 2010 Jun 11.

Abstract

Recent advances in optogenetic techniques have generated new tools for controlling neuronal activity, with a wide range of neuroscience applications. The most commonly used approach has been the optical activation of the light-gated ion channel channelrhodopsin-2 (ChR2). However, targeted single-cell-level optogenetic activation with temporal precessions comparable to the spike timing remained challenging. Here we report fast (< or = 1 ms), selective, and targeted control of neuronal activity with single-cell resolution in hippocampal slices. Using temporally focused laser pulses (TEFO) for which the axial beam profile can be controlled independently of its lateral distribution, large numbers of channels on individual neurons can be excited simultaneously, leading to strong (up to 15 mV) and fast (< or = 1 ms) depolarizations. Furthermore, we demonstrated selective activation of cellular compartments, such as dendrites and large presynaptic terminals, at depths up to 150 microm. The demonstrated spatiotemporal resolution and the selectivity provided by TEFO allow manipulation of neuronal activity, with a large number of applications in studies of neuronal microcircuit function in vitro and in vivo.

MeSH terms

  • Action Potentials / radiation effects
  • Animals
  • Cell Line
  • Channelrhodopsins
  • Dendrites / physiology
  • Dendrites / radiation effects
  • Electrophysiological Phenomena
  • Hippocampus / physiology
  • Hippocampus / radiation effects
  • Humans
  • Lasers
  • Mice
  • Mice, Transgenic
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / physiology
  • Nerve Tissue Proteins / radiation effects
  • Neurons / physiology*
  • Neurons / radiation effects*
  • Optical Phenomena
  • Patch-Clamp Techniques
  • Photic Stimulation
  • Photons
  • Presynaptic Terminals / physiology
  • Presynaptic Terminals / radiation effects
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Recombinant Fusion Proteins / radiation effects

Substances

  • Channelrhodopsins
  • Nerve Tissue Proteins
  • Recombinant Fusion Proteins