[RAPD analysis on the germplasm resources of Baphicacanthus cusia]

Zhong Yao Cai. 2010 Feb;33(2):183-6.
[Article in Chinese]

Abstract

Objective: To explore the genetic diversity of germplasm resources of Baphicacanthus cusia on molecular leve.

Methods: The molecular biological technique-random amplified polymorphic DNA (RAPD) were used.

Results: 104 random decamer primers were screened for RAPD fragments of Baphicacanthus cusia. 422 DNA bands were amplified by 12 primers, 55.69% products were found to be polymorphic. Base on UPGMA cluster analysis, a DNA molecular dendrogram was established to discuss the genetic diversity of the germplasm resources of Baphicacanthus cusia. The genetic differences are related to morphological differences on a certain extant, but not to geographic regions.

Conclusion: There actually existed much genetic diversity on molecular level among different natural populations of Baphicacanthus cusia.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acanthaceae / classification
  • Acanthaceae / genetics*
  • Acanthaceae / growth & development
  • Base Sequence
  • Cluster Analysis
  • DNA Primers / genetics
  • DNA, Plant / genetics*
  • Phylogeny
  • Plant Leaves / genetics
  • Plants, Medicinal / genetics*
  • Plants, Medicinal / growth & development
  • Polymorphism, Genetic*
  • Random Amplified Polymorphic DNA Technique / methods*

Substances

  • DNA Primers
  • DNA, Plant