Abstract
Procainamide, a widely used antiarrythmic, causes DNA hypomethylation in the human T cell line Jurkat, but the mechanism is unknown. We report that procainamide inhibits the DNA methyltransferase catalyzed transfer of methyl groups from S-adenosylmethionine to DNA, but has no effect on other known regulators of DNA methylation. Our results suggest that procainamide could inhibit cellular DNA methylation by inhibiting DNA methyltransferase activity.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Cell Line
-
DNA (Cytosine-5-)-Methyltransferases / metabolism*
-
Methylation
-
Phospholipids / metabolism
-
Procainamide / pharmacology*
-
Rats
-
S-Adenosylhomocysteine / metabolism*
-
S-Adenosylmethionine / metabolism*
-
T-Lymphocytes / enzymology*
Substances
-
Phospholipids
-
S-Adenosylmethionine
-
S-Adenosylhomocysteine
-
DNA (Cytosine-5-)-Methyltransferases
-
Procainamide