Abstract
pqqD is one of six genes required for PQQ production in Klebsiella pneumoniae. Herein, we demonstrate that PqqD interacts specifically with the radical SAM enzyme PqqE, causing a perturbation in the electronic environment around the [4Fe-4S](+) clusters. This interaction redirects the role for PqqD in PQQ biosynthesis.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Bacterial Proteins / chemistry*
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Bacterial Proteins / metabolism
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Free Radicals / chemistry
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Free Radicals / metabolism
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Klebsiella pneumoniae / enzymology
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PQQ Cofactor / biosynthesis*
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PQQ Cofactor / chemistry
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S-Adenosylmethionine / chemistry*
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S-Adenosylmethionine / metabolism
Substances
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Bacterial Proteins
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Free Radicals
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PQQ Cofactor
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S-Adenosylmethionine