Abstract
Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Biomarkers, Tumor / analysis*
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Biomarkers, Tumor / metabolism
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Biosensing Techniques / methods*
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Cell Death
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Cell Line, Tumor
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DNA Repair
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HeLa Cells
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Humans
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Luciferases / chemistry
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Luciferases / metabolism*
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Molecular Conformation
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Poly Adenosine Diphosphate Ribose / analysis*
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Poly Adenosine Diphosphate Ribose / metabolism
Substances
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Biomarkers, Tumor
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Poly Adenosine Diphosphate Ribose
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Luciferases