The purpose of this study was to evaluate the influence of different implant designs of total lumbar disc replacements on the segmental biomechanics of the lumbar spine. The unconstrained Charité, the semi-constrained Prodisc and a semi-constrained Prototype with more posterior centre of rotation than the Prodisc were tested in vitro using six human, lumbar spines L2-L5. The segmental lordosis was measured on plain radiographs and the range of motion (ROM) for all six degrees of freedom with a previously described spine tester. All prostheses were implanted at level L3-L4. Compared with the intact status all prostheses resulted in a significant increase of segmental lordosis (intact 5.1°; Charité 10.6°, p = 0.028; Prodisc 9.5°, p = 0.027; Prototype 8.9°, p = 0.028), significant increase of flexion/extension (intact 6.4°, Charité 11.3°, Prodisc 12.2°, Prototype 12.2°) and axial rotation (intact 1.3°, Charité 5.4°, Prodisc 3.9°, Prototype 4.2°). Lateral bending increased significantly only for the Charité (intact 7.7°; Charité 11.6°, p = 0.028; Prodisc 9.6°, Prototype 9.8°). The segmental lordosis after Prototype implantation was significantly lower compared with Charité (p = 0.024) and Prodisc (p = 0.044). No significant difference could be observed for segmental lordosis between Charité and Prodisc and for ROM between the two semi-constrained prosthesis Prodisc and Prototype. The axial rotation for the unconstrained Charité was significantly higher than for the semi-constrained prosthesis Prodisc and Prototype, flexion/extension and lateral bending did not differ. Summarizing, the unconstrained prosthesis design increased segmental lordosis and showed a tendency towards higher ROM for axial rotation/lateral bending and lower ROM for flexion/extension than a semi-constrained prosthesis. A more anterior centre of rotation in a semi-constrained prosthesis resulted in a higher increase of segmental lordosis after TDR than a semi-constrained prosthesis with more posterior centre of rotation. The location of the centre of rotation in a semi-constrained prosthesis did not alter the magnitude of ROM. Despite the different alterations of ROM and segmental lordosis due to implant design, these differences were negligible compared with the overall increase of ROM and segmental lordosis by the implantation of a TDR compared with the physiologic state.