Abstract
Regulated adhesion between cells and their environment is critical for normal cell migration. We have identified mutations in a gene encoding the Drosophila hydrogen peroxide (H₂O₂)-degrading enzyme Jafrac1, which lead to germ cell adhesion defects. During gastrulation, primordial germ cells (PGCs) associate tightly with the invaginating midgut primordium as it enters the embryo; however, in embryos from jafrac1 mutant mothers this association is disrupted, leaving some PGCs trailing on the outside of the embryo. We observed similar phenotypes in embryos from DE-cadherin/shotgun (shg) mutant mothers and were able to rescue the jafrac1 phenotype by increasing DE-cadherin levels. This and our biochemical evidence strongly suggest that Jafrac1-mediated reduction of H₂O₂ is required to maintain DE-cadherin protein levels in the early embryo. Our results present in vivo evidence of a peroxiredoxin regulating DE-cadherin-mediated adhesion.
Copyright © 2011 Elsevier Inc. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adherens Junctions / drug effects
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Adherens Junctions / metabolism
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Animals
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Cadherins / genetics
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Cadherins / metabolism*
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Cell Adhesion / drug effects
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Cell Aggregation / drug effects
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Cell Survival / drug effects
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Drosophila Proteins / genetics
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Drosophila Proteins / metabolism*
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Drosophila melanogaster / cytology*
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Drosophila melanogaster / embryology
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Drosophila melanogaster / enzymology*
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Drosophila melanogaster / metabolism
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Embryo, Nonmammalian / cytology
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Embryo, Nonmammalian / drug effects
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Embryo, Nonmammalian / metabolism
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Endocytosis / drug effects
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Gastrulation / drug effects
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Gene Expression Regulation / drug effects
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Germ Cells / cytology*
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Germ Cells / drug effects
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Germ Cells / enzymology*
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Hydrogen Peroxide / pharmacology
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Oxidative Stress / drug effects
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Peroxidases / metabolism*
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Protein Stability / drug effects
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Transcription, Genetic / drug effects
Substances
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Cadherins
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Drosophila Proteins
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shg protein, Drosophila
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Hydrogen Peroxide
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Jafrac1 protein, Drosophila
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Peroxidases