Correlation between the distribution of SP and CGRP immunopositive neurons in dorsal root ganglia and the afferent sensation of preputial frenulum

Abstract

The aim of this study was to explore the distribution of substance P (SP) and calcitonin gene-related peptide (CGRP) immunoreactive nerve terminals in the penis prepuce and the preputial frenulum. The possible correlation between SP- and CGRP-immunopositive neurons in dorsal root ganglia (DRG) and the afferent sensation of the penile preputial frenulum is also discussed. Immunohistochemistry showed SP- and CGRP-positive nerve terminals in the epidermal basal layer of the prepuce and frenulum in adult human males. The majority of the nerve terminals presented as bundles of different lengths and a few as enlarged nodosities. The density of SP- and CGRP-immunopositive nerve terminals in the preputial frenulum was significantly higher than those in the penis prepuce (P<0.01). Fluoro-Gold (FG) retrograde tracing method was used to trace the origin of nerve terminals in Sprague-Dawley rats. SP and CGRP immunofluorescence labeling was employed to detect the distribution of SP- and CGRP-immunoreactive neurons in DRG. FG retro-labeled neurons were localized in L(6) -DRG and S(1) -DRG. All the FG/SP and FG/CGRP double-labeled neurons were medium or small-sized. One-third of the FG-labeled neurons were SP-immunoreactive, and a half of them CGRP-immunoreactive in L(6) -DRG and S(1) -DRG, respectively. The FG/SP/CGRP-labeled neurons accounted for one fifth of the FG retro-labeled neurons. Taken together, these data suggest that the SP- and CGRP-immunopositive nerve fibers may participate in the transmission of afferent sensation in the preputial frenulum.