Interactions of taurine with GABAB binding sites in mouse brain

Neuropharmacology. 1990 Mar;29(3):243-7. doi: 10.1016/0028-3908(90)90008-f.

Abstract

The interactions of taurine with GABAB receptors were studied in membranes from the brain of the mouse by measuring the binding of [3H]baclofen and that of [3H]GABA in the presence of isoguvacine. Taurine displaced ligand binding to GABAB receptors concentration-dependently with an IC50 in the micromolar range. The effects of baclofen on the release of taurine and GABA from slices of cerebral cortex of the mouse were assessed using a superfusion system. Potassium-stimulated release of both [3H]taurine and [3H]GABA was unaffected by baclofen but potentiated by delta-aminovalerate. The enhancement of release of [3H]taurine by delta-aminovalerate was partially antagonized by baclofen, suggesting that baclofen-sensitive receptors could modify the release.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / pharmacology
  • Amino Acids, Neutral*
  • Animals
  • Baclofen / pharmacology
  • Brain Chemistry / drug effects*
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / metabolism
  • Female
  • Guinea Pigs
  • In Vitro Techniques
  • Kinetics
  • Male
  • Potassium / pharmacology
  • Receptors, GABA-A / drug effects*
  • Synaptosomes / drug effects
  • Synaptosomes / metabolism
  • Taurine / pharmacology*

Substances

  • Amino Acids
  • Amino Acids, Neutral
  • Receptors, GABA-A
  • Taurine
  • 5-aminovaleric acid
  • Baclofen
  • Potassium