Detection of Porphyromonas gulae from subgingival biofilms of dogs with and without periodontitis

Gerusa Neyla Andrade Senhorinho; Viviane Nakano; Chengxu Liu; Yuli Song; Sydney M Finegold; Mario J Avila-Campos

doi:10.1016/j.anaerobe.2011.06.002

Detection of Porphyromonas gulae from subgingival biofilms of dogs with and without periodontitis

Anaerobe. 2011 Oct;17(5):257-8. doi: 10.1016/j.anaerobe.2011.06.002. Epub 2011 Jun 23.

Authors

Gerusa Neyla Andrade Senhorinho¹, Viviane Nakano, Chengxu Liu, Yuli Song, Sydney M Finegold, Mario J Avila-Campos

Affiliation

¹ Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Brazil.

PMID: 21723404
DOI: 10.1016/j.anaerobe.2011.06.002

Abstract

A rapid PCR approach was developed to detect Porphyromonas gulae strains from subgingival samples of dogs with and with periodontitis. The presence of P. gulae was observed in 92% and 56%, respectively, in dogs with and without periodontitis. The new primer pair was specific to detect this microorganism, and this technique could be used to evaluate a correlation between periodontitis and P. gulae in companion animals.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacteroidaceae Infections / microbiology
Bacteroidaceae Infections / veterinary*
Biofilms*
DNA Primers
Dog Diseases / microbiology*
Dogs
Gingiva / microbiology*
Periodontitis / microbiology
Periodontitis / veterinary*
Polymerase Chain Reaction / methods*
Porphyromonas / classification
Porphyromonas / genetics
Porphyromonas / isolation & purification*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA

Substances

DNA Primers
RNA, Ribosomal, 16S