1α,25-Dihydroxyvitamin D₃ inhibits neutrophil recruitment in hamster model of acute lung injury

Steroids. 2011 Nov;76(12):1305-9. doi: 10.1016/j.steroids.2011.06.009. Epub 2011 Jul 2.

Abstract

The chemokine interleukin-8 (IL-8) is involved in the pathogenesis of acute lung injury (ALI). Although several studies have reported that 1α,25-dihydroxyvitamin D(3) (1α,25(OH)(2)D(3)) suppresses IL-8 production in vitro and in vivo, 1α,25(OH)(2)D(3) has not been demonstrated to be effective in an animal model of ALI. Here, we determined its effects of 1α,25(OH)(2)D(3) in a hamster model where ALI was induced by lipopolysaccharide (LPS) inhalation. 1α,25(OH)(2)D(3) inhibited neutrophil recruitment in the lung by approximately 40% without increasing plasma calcium concentration, while it did not inhibit monocyte recruitment. Our findings show that vitamin D(3) analogues may be suitable as novel anti-inflammatory agents for ALI.

MeSH terms

  • Acute Lung Injury / chemically induced
  • Acute Lung Injury / immunology*
  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Bronchoalveolar Lavage Fluid / cytology
  • Calcitriol / pharmacology*
  • Calcium / blood
  • Cricetinae
  • Dexamethasone / pharmacology
  • Disease Models, Animal
  • Lipopolysaccharides / toxicity
  • Male
  • Mesocricetus
  • Monocytes / drug effects
  • Monocytes / immunology
  • Neutrophil Infiltration / drug effects*
  • Neutrophils / drug effects
  • Neutrophils / immunology

Substances

  • Anti-Inflammatory Agents
  • Lipopolysaccharides
  • Dexamethasone
  • Calcitriol
  • Calcium