Concurrent proinflammatory and apoptotic activity of a Helicobacter pylori protein (HP986) points to its role in chronic persistence

PLoS One. 2011;6(7):e22530. doi: 10.1371/journal.pone.0022530. Epub 2011 Jul 15.

Abstract

Helicobacter pylori induces cytokine mediated changes in gastroduodenal pathophysiology, wherein, the activated macrophages at the sub-mucosal space play a central role in mounting innate immune response against the antigens. The bacterium gains niche through persistent inflammation and local immune-suppression causing peptic ulcer disease or chronic gastritis; the latter being a significant risk factor for the development of gastric adenocarcinoma. What favors persistence of H. pylori in the gastric niches is not clearly understood. We report detailed characterization of a functionally unknown gene (HP986), which was detected in patient isolates associated with peptic ulcer and gastric carcinoma. Expression and purification of recombinant HP986 (rHP986) revealed a novel, ∼29 kDa protein in biologically active form which associates with significant levels of humoral immune responses in diseased individuals (p<0.001). Also, it induced significant levels of TNF-α and Interleukin-8 in cultured human macrophages concurrent to the translocation of nuclear transcription factor-κB (NF-κB). Further, the rHP986 induced apoptosis of cultured macrophages through a Fas mediated pathway. Dissection of the underlying signaling mechanism revealed that rHP986 induces both TNFR1 and Fas expression to lead to apoptosis. We further demonstrated interaction of HP986 with TNFR1 through computational and experimental approaches. Independent proinflammatory and apoptotic responses triggered by rHP986 as shown in this study point to its role, possibly as a survival strategy to gain niche through inflammation and to counter the activated macrophages to avoid clearance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Apoptosis*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / metabolism*
  • Gastrointestinal Diseases / metabolism
  • Gastrointestinal Diseases / microbiology
  • Gastrointestinal Diseases / pathology
  • Genetic Loci / genetics
  • Helicobacter Infections / metabolism*
  • Helicobacter Infections / microbiology
  • Helicobacter Infections / pathology
  • Helicobacter pylori / isolation & purification
  • Helicobacter pylori / metabolism*
  • Humans
  • Immunity, Humoral
  • Inflammation Mediators / metabolism*
  • Interleukin-8 / metabolism
  • Models, Biological
  • Molecular Sequence Data
  • NF-kappa B / metabolism
  • Protein Binding
  • Receptors, Tumor Necrosis Factor, Type I / metabolism
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Sequence Analysis, Protein
  • Time Factors
  • Tumor Necrosis Factor-alpha / metabolism
  • fas Receptor / metabolism

Substances

  • Bacterial Proteins
  • FAS protein, human
  • Inflammation Mediators
  • Interleukin-8
  • NF-kappa B
  • Receptors, Tumor Necrosis Factor, Type I
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • fas Receptor