Analyzing multiclonality of Staphylococcus aureus in clinical diagnostics using spa-based denaturing gradient gel electrophoresis

J Clin Microbiol. 2011 Oct;49(10):3647-8. doi: 10.1128/JCM.00389-11. Epub 2011 Aug 10.

Abstract

We present a novel denaturing gradient gel electrophoresis (DGGE) method which characterizes multiclonal communities of Staphylococcus aureus. The spa PCR-based DGGE method simultaneously separates strains that differ in only one base, thereby revealing multiclonal colonization and infections.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coinfection / microbiology*
  • DNA, Bacterial / genetics
  • Denaturing Gradient Gel Electrophoresis / methods*
  • Humans
  • Molecular Typing / methods*
  • Polymerase Chain Reaction / methods
  • Staphylococcal Infections / microbiology*
  • Staphylococcal Protein A / genetics*
  • Staphylococcus aureus / classification*
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / isolation & purification

Substances

  • DNA, Bacterial
  • Staphylococcal Protein A