Abstract
miR-132 is a CREB-induced microRNA that is involved in dendritic spine plasticity. We found that visual experience regulated histone post-translational modifications at a CRE locus that is important for miR-212 and miR-132 cluster transcription, and regulated miR-132 expression in the visual cortex of juvenile mice. Monocular deprivation reduced miR-132 expression in the cortex contralateral to the deprived eye. Counteracting this miR-132 reduction with an infusion of chemically modified miR-132 mimic oligonucleotides completely blocked ocular dominance plasticity.
© 2011 Nature America, Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Action Potentials / drug effects
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Action Potentials / physiology
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Age Factors
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Analysis of Variance
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Animals
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Animals, Newborn
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CREB-Binding Protein / genetics
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CREB-Binding Protein / metabolism
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Chromatin Immunoprecipitation
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Critical Period, Psychological
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Dominance, Ocular / drug effects
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Dominance, Ocular / physiology*
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Evoked Potentials, Visual / drug effects
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Gene Expression Regulation, Developmental / physiology*
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Green Fluorescent Proteins / genetics
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Histones / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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MicroRNAs / chemistry
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MicroRNAs / genetics
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MicroRNAs / metabolism*
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NIH 3T3 Cells
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Neuronal Plasticity / drug effects
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Neuronal Plasticity / physiology*
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Neurons / drug effects
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Neurons / physiology*
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Oligonucleotides / pharmacology
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Photic Stimulation / methods
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Protein Processing, Post-Translational / physiology
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Sensory Deprivation
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Visual Cortex / cytology*
Substances
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Histones
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MIRN132 microRNA, mouse
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MIRN212 microRNA, mouse
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MicroRNAs
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Oligonucleotides
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Green Fluorescent Proteins
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CREB-Binding Protein