Abstract
A 2.1 kb DNA segment carrying the purine-cytosine permease gene (FCY2) of Saccharomyces cerevisiae was sequenced, the primary structure of the protein (533 amino acids) deduced and a folding pattern in the membrane is proposed for the permease protein. Expression of the FCY2 gene product requires a functional secretory pathway and is reduced in mnn9, a mutant strain deficient in outer chain glycosylation. The FCY2 gene was mapped on the right arm of chromosome V close to the HIS1 gene.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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DNA, Fungal / biosynthesis*
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Gene Amplification
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Genes, Fungal*
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Glycosylation
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Membrane Transport Proteins*
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Molecular Sequence Data
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Molecular Weight
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Mutation
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Nucleobase Transport Proteins
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Protein Conformation
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Restriction Mapping
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
Substances
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Carrier Proteins
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DNA, Fungal
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FCY2 protein, S cerevisiae
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Membrane Transport Proteins
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Nucleobase Transport Proteins
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Saccharomyces cerevisiae Proteins