A yeast-Escherichia coli shuttle vector containing the M13 origin of replication

N Rhodes; M Company; B Errede

doi:10.1016/0147-619x(90)90036-c

A yeast-Escherichia coli shuttle vector containing the M13 origin of replication

Plasmid. 1990 Mar;23(2):159-62. doi: 10.1016/0147-619x(90)90036-c.

Authors

N Rhodes¹, M Company, B Errede

Affiliation

¹ Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290.

PMID: 2194231
DOI: 10.1016/0147-619x(90)90036-c

Abstract

A yeast-Escherichia coli shuttle vector containing the M13 origin of replication has been constructed. This vector allows selection and replication in both Saccharomyces cerevisiae and E. coli, as well as single-stranded packaging from E. coli upon infection with a helper phage. The presence of a polylinker with various unique restriction sites facilitates the cloning of desired genes.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Base Sequence
Cloning, Molecular
Coliphages / genetics*
DNA Replication*
Escherichia coli / genetics*
Escherichia coli / growth & development
Genetic Vectors*
Mitosis
Molecular Sequence Data
Plasmids*
Restriction Mapping
Saccharomyces cerevisiae / cytology
Saccharomyces cerevisiae / genetics*

Associated data

GENBANK/M55338
GENBANK/M55339

Grants and funding

GM-30619/GM/NIGMS NIH HHS/United States