RNA targets of wild-type and mutant FET family proteins

Nat Struct Mol Biol. 2011 Nov 13;18(12):1428-31. doi: 10.1038/nsmb.2163.

Abstract

FUS, EWSR1 and TAF15, constituting the FET protein family, are abundant, highly conserved RNA-binding proteins with important roles in oncogenesis and neuronal disease, yet their RNA targets and recognition elements are unknown. Using PAR-CLIP, we defined global RNA targets for all human FET proteins and two ALS-causing human FUS mutants. FET members showed similar binding profiles, whereas FUS mutants showed a drastically altered binding pattern, consistent with changes in subcellular localization.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyotrophic Lateral Sclerosis / genetics
  • Base Sequence
  • Binding Sites
  • Calmodulin-Binding Proteins / chemistry
  • Calmodulin-Binding Proteins / metabolism*
  • HEK293 Cells
  • Humans
  • Mutation
  • RNA, Messenger / chemistry
  • RNA, Messenger / metabolism*
  • RNA-Binding Protein EWS
  • RNA-Binding Protein FUS / chemistry
  • RNA-Binding Protein FUS / metabolism*
  • RNA-Binding Proteins / chemistry
  • RNA-Binding Proteins / metabolism*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • TATA-Binding Protein Associated Factors / chemistry
  • TATA-Binding Protein Associated Factors / metabolism*

Substances

  • Calmodulin-Binding Proteins
  • EWSR1 protein, human
  • RNA, Messenger
  • RNA-Binding Protein EWS
  • RNA-Binding Protein FUS
  • RNA-Binding Proteins
  • TAF15 protein, human
  • TATA-Binding Protein Associated Factors