Abstract
The sco6545 gene of Streptomyces coelicolor A3(2) was nominated as a putative cellulase with 863 mature-form amino acids (90.58 kDa). We overexpressed and purified Sco6545 and demonstrated that the protein is not a cellulase but a xyloglucan-specific glycosyl hydrolase which cleaves xyloglucan at unbranched glucose residues.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cloning, Molecular
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Gene Expression
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Glucans / metabolism
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Glycoside Hydrolases / chemistry
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Glycoside Hydrolases / genetics*
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Glycoside Hydrolases / isolation & purification
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Glycoside Hydrolases / metabolism*
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Hydrolases
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Molecular Weight
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Streptomyces coelicolor / enzymology*
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Streptomyces coelicolor / genetics
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Streptomyces coelicolor / metabolism
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Xylans / metabolism
Substances
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Glucans
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Recombinant Proteins
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Xylans
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xyloglucan
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Hydrolases
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Glycoside Hydrolases
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xyloglucan endo(1-4)-beta-D-glucanase