Identification and characterization of a xyloglucan-specific family 74 glycosyl hydrolase from Streptomyces coelicolor A3(2)

Appl Environ Microbiol. 2012 Jan;78(2):607-11. doi: 10.1128/AEM.06482-11. Epub 2011 Nov 18.

Abstract

The sco6545 gene of Streptomyces coelicolor A3(2) was nominated as a putative cellulase with 863 mature-form amino acids (90.58 kDa). We overexpressed and purified Sco6545 and demonstrated that the protein is not a cellulase but a xyloglucan-specific glycosyl hydrolase which cleaves xyloglucan at unbranched glucose residues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • Gene Expression
  • Glucans / metabolism
  • Glycoside Hydrolases / chemistry
  • Glycoside Hydrolases / genetics*
  • Glycoside Hydrolases / isolation & purification
  • Glycoside Hydrolases / metabolism*
  • Hydrolases
  • Molecular Weight
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Streptomyces coelicolor / enzymology*
  • Streptomyces coelicolor / genetics
  • Streptomyces coelicolor / metabolism
  • Xylans / metabolism

Substances

  • Glucans
  • Recombinant Proteins
  • Xylans
  • xyloglucan
  • Hydrolases
  • Glycoside Hydrolases
  • xyloglucan endo(1-4)-beta-D-glucanase