Evaluation of xTAG Respiratory Viral Panel FAST and xTAG Human Parainfluenza Virus Analyte-Specific Reagents for detection of human parainfluenza viruses in respiratory specimens

Suresh B Selvaraju; Rangaraj Selvarangan

doi:10.1016/j.diagmicrobio.2011.11.005

Evaluation of xTAG Respiratory Viral Panel FAST and xTAG Human Parainfluenza Virus Analyte-Specific Reagents for detection of human parainfluenza viruses in respiratory specimens

Diagn Microbiol Infect Dis. 2012 Mar;72(3):278-81. doi: 10.1016/j.diagmicrobio.2011.11.005. Epub 2011 Dec 29.

Authors

Suresh B Selvaraju¹, Rangaraj Selvarangan

Affiliation

¹ Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, Kansas City, MO 64108, USA.

Abstract

The multiplex xTAG(®) Respiratory Viral Panel FAST (RVP FAST) research-use-only assay and xTAG(®) Human Parainfluenza Virus Analyte-Specific Reagent (HPIV-ASR) assay were evaluated with 99 culture-confirmed human parainfluenza virus (HPIV)-positive and -negative specimens and found to have analytical sensitivities of 95.2% and 100% and specificities of 98.3% and 96.6%, respectively. Since the in vitro diagnostic (IVD) version of the RVP FAST assay does not include HPIVs, the HPIV-ASR assay can be tested in parallel with RVP FAST-IVD for optimal detection of HPIVs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Paramyxoviridae / genetics
Paramyxoviridae / isolation & purification*
Paramyxoviridae Infections / diagnosis*
Reagent Kits, Diagnostic*
Reproducibility of Results
Respiratory System / virology
Sensitivity and Specificity

Substances

Reagent Kits, Diagnostic