BMP2/7 heterodimer is a stronger inducer of bone regeneration in peri-implant bone defects model than BMP2 or BMP7 homodimer

Dent Mater J. 2012;31(2):239-48. doi: 10.4012/dmj.2011-191. Epub 2012 Mar 23.

Abstract

This study aimed to compare the effects of bone morphogenetic protein BMP2/7 heterodimer and BMP homodimers on bone regeneration in bone defects model. Identical peri-implant bone defects model were created using proper controls on the frontal skull in 18 minipigs. Collagen sponges with low-dose (30 ng/mL) BMP2/7 heterodimer, BMP2 or BMP7 homodimer were filled in the defects. New bone formation and the expression of type I collagen (Col1), alkaline phosphatase (ALP) and osteocalcin (OCN) were evaluated after 2, 3, and 6 weeks of implantation. BMP2/7 resulted in significantly higher new bone areas percentage in the defect region than BMP2 and BMP7 (p<0.05). Immunohistochemical staining of Col1, ALP and OCN was stronger in BMP2/7 group than BMP2, BMP7 and control group (p<0.05). These results demonstrate that BMP2/7 heterodimer is a stronger inducer of osteoblastogenesis and could be applied at low dose to reduce the cost and side effects of BMP homodimers.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / biosynthesis
  • Alveolar Bone Loss / etiology
  • Alveolar Bone Loss / surgery
  • Animals
  • Bone Morphogenetic Protein 2 / administration & dosage
  • Bone Morphogenetic Protein 2 / chemistry*
  • Bone Morphogenetic Protein 2 / pharmacology
  • Bone Morphogenetic Protein 7 / administration & dosage
  • Bone Morphogenetic Protein 7 / chemistry*
  • Bone Morphogenetic Protein 7 / pharmacology
  • Bone Regeneration / drug effects*
  • Collagen
  • Collagen Type I / biosynthesis
  • Dental Implants / adverse effects
  • Dimerization
  • Drug Carriers
  • Female
  • Male
  • Osteoblasts / metabolism
  • Osteocalcin / biosynthesis
  • Peri-Implantitis / etiology
  • Peri-Implantitis / surgery*
  • Protein Multimerization*
  • Random Allocation
  • Recombinant Proteins
  • Skull / surgery
  • Swine
  • Swine, Miniature
  • Tissue Engineering

Substances

  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Protein 7
  • Collagen Type I
  • Dental Implants
  • Drug Carriers
  • Recombinant Proteins
  • Osteocalcin
  • Collagen
  • Alkaline Phosphatase