Abstract
This study aimed to compare the effects of bone morphogenetic protein BMP2/7 heterodimer and BMP homodimers on bone regeneration in bone defects model. Identical peri-implant bone defects model were created using proper controls on the frontal skull in 18 minipigs. Collagen sponges with low-dose (30 ng/mL) BMP2/7 heterodimer, BMP2 or BMP7 homodimer were filled in the defects. New bone formation and the expression of type I collagen (Col1), alkaline phosphatase (ALP) and osteocalcin (OCN) were evaluated after 2, 3, and 6 weeks of implantation. BMP2/7 resulted in significantly higher new bone areas percentage in the defect region than BMP2 and BMP7 (p<0.05). Immunohistochemical staining of Col1, ALP and OCN was stronger in BMP2/7 group than BMP2, BMP7 and control group (p<0.05). These results demonstrate that BMP2/7 heterodimer is a stronger inducer of osteoblastogenesis and could be applied at low dose to reduce the cost and side effects of BMP homodimers.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / biosynthesis
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Alveolar Bone Loss / etiology
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Alveolar Bone Loss / surgery
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Animals
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Bone Morphogenetic Protein 2 / administration & dosage
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Bone Morphogenetic Protein 2 / chemistry*
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Bone Morphogenetic Protein 2 / pharmacology
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Bone Morphogenetic Protein 7 / administration & dosage
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Bone Morphogenetic Protein 7 / chemistry*
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Bone Morphogenetic Protein 7 / pharmacology
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Bone Regeneration / drug effects*
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Collagen
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Collagen Type I / biosynthesis
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Dental Implants / adverse effects
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Dimerization
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Drug Carriers
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Female
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Male
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Osteoblasts / metabolism
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Osteocalcin / biosynthesis
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Peri-Implantitis / etiology
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Peri-Implantitis / surgery*
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Protein Multimerization*
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Random Allocation
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Recombinant Proteins
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Skull / surgery
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Swine
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Swine, Miniature
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Tissue Engineering
Substances
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Bone Morphogenetic Protein 2
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Bone Morphogenetic Protein 7
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Collagen Type I
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Dental Implants
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Drug Carriers
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Recombinant Proteins
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Osteocalcin
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Collagen
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Alkaline Phosphatase