Triple-color super-resolution imaging of live cells: resolving submicroscopic receptor organization in the plasma membrane

Angew Chem Int Ed Engl. 2012 May 14;51(20):4868-71. doi: 10.1002/anie.201200853. Epub 2012 Apr 5.

Abstract

In living color: efficient intracellular covalent labeling of proteins with a photoswitchable dye using the HaloTag for dSTORM super-resolution imaging in live cells is described. The dynamics of cellular nanostructures at the plasma membrane were monitored with a time resolution of a few seconds. In combination with dual-color FPALM imaging, submicroscopic receptor organization within the context of the membrane skeleton was resolved.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / chemistry
  • Actins / metabolism
  • Cell Membrane / chemistry*
  • Cell Membrane / metabolism
  • Cytoskeleton / chemistry
  • Cytoskeleton / metabolism
  • Fluorescent Dyes / chemistry
  • Green Fluorescent Proteins / chemistry*
  • Green Fluorescent Proteins / metabolism
  • HeLa Cells
  • Humans
  • Microscopy, Fluorescence / methods*
  • Nanostructures / chemistry
  • Transfection

Substances

  • Actins
  • Fluorescent Dyes
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins