Nucleotide sequence of the rep gene of staphylococcal plasmid pCW7

Plasmid. 1990 Jul;24(1):74-80. doi: 10.1016/0147-619x(90)90027-a.

Abstract

Previous heteroduplex mapping studies showed that staphylococcal plasmid pCW7 belongs to the pT181 family of small antibiotic resistance plasmids which replicate by a rolling-circle mechanism. Replication in each case is initiated at the plasmid origin (ori) by a plasmid-encoded protein. Rep, which makes a sequence-specific single-stranded nick to form a covalent Rep-ori replication intermediate. A comparison of sequencing results for the repN gene of pCW7 with data for the products of five other homologous rep genes allows a prediction to be made of the segments of the primary structure of Rep which are likely to be responsible for plasmid-specific recognition of the ori region by each Rep protein.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Base Sequence
  • DNA, Bacterial / genetics
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Plasmids*
  • Sequence Homology, Nucleic Acid
  • Staphylococcus aureus / genetics*

Substances

  • Bacterial Proteins
  • DNA, Bacterial

Associated data

  • GENBANK/J03323