Xiangella phaseoli gen. nov., sp. nov., a member of the family Micromonosporaceae

Int J Syst Evol Microbiol. 2013 Jun;63(Pt 6):2138-2145. doi: 10.1099/ijs.0.045732-0. Epub 2012 Oct 26.

Abstract

A novel endophytic actinomycete, designated strain NEAU-J5(T) was isolated from roots of snap bean (Phaseolus vulgaris L.). Comparative analysis of the 16S rRNA gene sequence indicated that NEAU-J5(T) is phylogenetically related to members of the family Micromonosporaceae. The whole-cell sugars were galactose, mannose and glucose. The predominant menaquinones were MK-9(H4) and MK-9(H6). The major fatty acids were C16:0, C18:0, C17:1ω7c, iso-C15:0 and C17:0. The phospholipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and phosphatidylinositol mannoside. The DNA G+C content was 72.2 mol%. On the basis of the morphological and chemotaxonomic characteristics, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain NEAU-J5(T) represents a novel species of a new genus within the family Micromonosporaceae, for which the name Xiangella phaseoli gen. nov., sp. nov. is proposed. The type strain of Xiangella phaseoli is strain NEAU-J5(T) (=CGMCC 4.7038(T)=DSM 45730(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition
  • China
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Micromonosporaceae / classification*
  • Micromonosporaceae / genetics
  • Micromonosporaceae / isolation & purification
  • Molecular Sequence Data
  • Phaseolus / microbiology*
  • Phospholipids / analysis
  • Phylogeny*
  • Plant Roots / microbiology*
  • RNA, Ribosomal, 16S / genetics

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/JQ073732