Abstract
Conformational analysis: Capillary electrophoresis (CE) allows for the rapid separation of slowly interconverting protein conformers. Kinetic analysis (k(open), k(closed), and K(d)) of electropherograms in the presence and absence of effector ligands allows the measurement of kinetic and thermodynamic constants associated with conformational changes and ligand binding.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Allosteric Regulation
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Calcium / chemistry
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Electrophoresis, Capillary*
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GTP-Binding Proteins / chemistry
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GTP-Binding Proteins / metabolism
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Guanosine Diphosphate / chemistry
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Guanosine Diphosphate / metabolism
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Humans
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Kinetics
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Ligands
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Protein Glutamine gamma Glutamyltransferase 2
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Protein Structure, Tertiary
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Proteins / chemistry*
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Proteins / metabolism
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Solutions / chemistry*
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Thermodynamics
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Transglutaminases / chemistry
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Transglutaminases / metabolism
Substances
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Ligands
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Proteins
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Solutions
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Guanosine Diphosphate
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Protein Glutamine gamma Glutamyltransferase 2
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Transglutaminases
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GTP-Binding Proteins
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Calcium