Loss of full knee extension following anterior cruciate ligament surgery has been shown to impair knee function. However, there can be significant difficulties in accurately and reproducibly measuring a fixed flexion of the knee. We studied the interobserver and the intraobserver reliabilities of a novel, smartphone accelerometer-based, knee goniometer and compared it with a long-armed conventional goniometer for the assessment of fixed flexion knee deformity. Five healthy male volunteers (age range 30 to 40 years) were studied. Measurements of knee flexion angle were made with a telescopic-armed goniometer (Lafayette Instrument, Lafayette, IN) and compared with measurements using the smartphone (iPhone 3GS, Apple Inc., Cupertino, CA) knee goniometer using a novel trigonometric technique based on tibial inclination. Bland-Altman analysis of validity and reliability including statistical analysis of correlation by Pearson's method was undertaken. The iPhone goniometer had an interobserver correlation (r) of 0.994 compared with 0.952 for the Lafayette. The intraobserver correlation was r = 0.982 for the iPhone (compared with 0.927). The datasets from the two instruments correlate closely (r = 0.947) are proportional and have mean difference of only -0.4 degrees (SD 3.86 degrees). The Lafayette goniometer had an intraobserver reliability +/- 9.6 degrees. The interobserver reliability was +/- 8.4 degrees. By comparison the iPhone had an interobserver reliability +/- 2.7 degrees and an intraobserver reliability +/- 4.6 degrees. We found the iPhone goniometer to be a reliable tool for the measurement of subtle knee flexion in the clinic setting.