Studies on the spectrophotometric determination of DNA hybridization from renaturation rates

V A Huss; H Festl; K H Schleifer

doi:10.1016/S0723-2020(83)80048-4

Studies on the spectrophotometric determination of DNA hybridization from renaturation rates

Syst Appl Microbiol. 1983;4(2):184-92. doi: 10.1016/S0723-2020(83)80048-4.

Authors

V A Huss¹, H Festl, K H Schleifer

Affiliation

¹ Lehrstuhl für Mikrobiologie, Technische Universität München, 8000 München 2, Federal Republic of Germany.

PMID: 23194591
DOI: 10.1016/S0723-2020(83)80048-4

Abstract

The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined. Several parameters such as incubation temperature, incubation time, DNA concentration and DNA fragment length were checked and the optimal conditions established. Hybridization data of several anaerobic Gram-positive cocci were compared with values obtained by the membrane filter technique. The agreement is excellent above a degree of binding of 25-30%.