Abstract
We report an optogenetic method based on Arabidopsis thaliana cryptochrome 2 for rapid and reversible protein oligomerization in response to blue light. We demonstrated its utility by photoactivating the β-catenin pathway, achieving a transcriptional response higher than that obtained with the natural ligand Wnt3a. We also demonstrated the modularity of this approach by photoactivating RhoA with high spatiotemporal resolution, thereby suggesting a previously unknown mode of activation for this Rho GTPase.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Arabidopsis Proteins / radiation effects
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Blotting, Western
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Cell Culture Techniques
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Cell Nucleus / metabolism
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Cell Nucleus / radiation effects
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Cryptochromes / genetics
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Cryptochromes / metabolism*
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Cryptochromes / radiation effects
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Cytoplasm / metabolism
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Cytoplasm / radiation effects
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Enzyme-Linked Immunosorbent Assay
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Fluorescence Recovery After Photobleaching
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HEK293 Cells
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Humans
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Light
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Light Signal Transduction
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Luminescent Proteins / metabolism
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Luminescent Proteins / radiation effects
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Mice
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NIH 3T3 Cells
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Protein Multimerization* / radiation effects
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Recombinant Fusion Proteins / metabolism
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Recombinant Fusion Proteins / radiation effects
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Red Fluorescent Protein
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Transcription, Genetic
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Transfection
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Wnt Signaling Pathway* / radiation effects
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Wnt3A Protein / genetics
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Wnt3A Protein / metabolism*
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Wnt3A Protein / radiation effects
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beta Catenin / genetics
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beta Catenin / metabolism*
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beta Catenin / radiation effects
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rhoA GTP-Binding Protein / genetics
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rhoA GTP-Binding Protein / metabolism*
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rhoA GTP-Binding Protein / radiation effects
Substances
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Arabidopsis Proteins
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CRY2 protein, Arabidopsis
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Cryptochromes
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Luminescent Proteins
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Recombinant Fusion Proteins
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Wnt3A Protein
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beta Catenin
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rhoA GTP-Binding Protein