Abstract
An effective approach, using the plasmid YCplac33-GHK, is developed to construct a ploidy series of Saccharomyces cerevisiae. YCplac33-GHK harbors the HO gene under the control of galactose-inducible promoter and KanMX4 as the selective marker. The simple method can solve the problem of industrial applications of strains with resistance genes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Deoxyribonucleases, Type II Site-Specific / genetics
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Genes, Fungal
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Plasmids / genetics*
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Ploidies*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins / genetics
Substances
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Saccharomyces cerevisiae Proteins
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HO protein, S cerevisiae
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Deoxyribonucleases, Type II Site-Specific