Abstract
Two recent papers in Science illustrate how the prokaryotic CRISPR-Cas immune system machinery, which typically targets invasive genetic elements such as viruses and plasmids, can be converted into a sophisticated molecular tool for next-generation human genome editing. The versatile Cas9 RNA-guided endonuclease can be readily reprogrammed using customizable small RNAs for sequence-specific single- or double-stranded DNA cleavage.
MeSH terms
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CRISPR-Cas Systems / genetics*
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Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
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DNA Restriction Enzymes
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Endonucleases
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Genetic Engineering
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Humans
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Plasmids / genetics
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RNA / genetics
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RNA / metabolism
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RNA Editing / genetics*
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RNA, Small Interfering / genetics
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RNA, Small Untranslated
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Viruses / genetics
Substances
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RNA, Small Interfering
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RNA
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Endonucleases
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DNA Restriction Enzymes
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RNA, Small Untranslated