The aim of this study was to compare positive placental alpha-microglobulin-1 (PAMG-1) test rates with clinical significance of pre-term pregnant women, intact membrane with and without uterine contraction. A prospective analytic study was performed including 100 pre-term pregnant women with intact membranes. Patients were classified into two groups, patients with uterine contraction (n = 50) and patients without contraction (n = 50). Conventional standard methods were performed to exclude rupture of membranes. PAMG-1 test was performed. Positive results of the tests and clinical significance (including time from test to delivery, route of delivery and outcomes of the fetuses) were determined. PAMG-1 positive rate was 10% (5/50) and 0% in contraction group and no contraction group, respectively (p = 0.028). Gestational age at delivery and test-to-delivery interval were less in contraction group than those in no-contraction group. When compared between positive test (n = 5) and negative test (n = 45) in pre-term contraction. Test-to-delivery interval was shorter positive group (20 vs. 720 h, p = 0.025). In conclusion, in pre-term pregnant women with intact membranes, PAMG-1 test rates are more positive in cases of uterine contraction. Pre-term pregnant women in labour with a positive PAMG-1 test had a significantly shorter test-to-delivery interval than those with a negative test.