Influenza NS1 interacts with p53 and alters its binding to p53-responsive genes, in a promoter-dependent manner

FEBS Lett. 2013 Sep 17;587(18):2965-71. doi: 10.1016/j.febslet.2013.08.006. Epub 2013 Aug 13.

Abstract

The interplay between influenza A viruses (IAV) and p53 has only been reported in a limited number of studies, mainly focusing on the antiviral role of p53. We investigated the impact of IAV infection on p53 stability and transcriptional activity. Our results indicate that IAV-induced stabilization of p53 only partially correlates with modulation of p53 transcriptional activity measured during infection. Moreover, we show that the viral non-structural protein 1 (NS1) is able to inhibit p53 transcriptional activity, in a promoter-dependent manner. Based on these data, we propose that NS1 may contribute to p53-mediated cell fate decision during IAV infection.

Keywords: Influenza virus; Non-structural protein NS1; Orthomyxoviridae; Transcription factor; p53.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Cell Line
  • Dogs
  • Host-Pathogen Interactions
  • Humans
  • Influenza A Virus, H3N2 Subtype / genetics
  • Influenza A Virus, H3N2 Subtype / metabolism*
  • Madin Darby Canine Kidney Cells
  • Promoter Regions, Genetic
  • Protein Binding
  • Protein Stability
  • Transcription, Genetic*
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / metabolism*
  • Viral Nonstructural Proteins / genetics
  • Viral Nonstructural Proteins / metabolism*

Substances

  • INS1 protein, influenza virus
  • Tumor Suppressor Protein p53
  • Viral Nonstructural Proteins